FCCV Monographs /Yeast,Autolyzed/507

FCCV Monographs /Yeast,Autolyzed/507
0.75mg/mLtoanotherconicalflask.Add1mLof Fehling’s
SolutionA andof Fehling’sSolutionB (see CupricTartrateTS,
Alkaline,under SolutionsandIndicators)toeachflask,heatto
boiling,andcool.Thesamplesolutionislessturbidthanthe
dextrosesolution,whichformsared-brownprecipitate.
ResidueonIgnition Determineasdirectedunder Residue
onIgnition,AppendixIIC,ignitinga2-gsample.
Water Determineasdirectedunder WaterDetermination,
AppendixIIB.
PackagingandStorage Storeinwell-closedcontainersin
adryplace.
Yeast,Autolyzed
AutolyzedYeast
DESCRIPTION
Yeast,Autolyzed,occursingranular,powdered,flake,or
pasteform.Itistheconcentrated,nonextracted,partiallysolu-
bledigestobtainedfromfood-gradeyeasts.Solubilizationis
accomplishedbyenzymehydrolysisorautolysisofyeastcells.
Food-gradesaltsandenzymesmaybeadded.Yeast,Auto-
lyzedcontainsbothsolubleandinsolublecomponentsderived
fromthewholeyeastcell.Itiscomposedprimarilyofamino
acids,peptides,proteins,carbohydrates,fats,andsalts.
Function Flavoringagent;flavorenhancer;proteinsource;
binder.
REQUIREMENTS
Note:Performallanalysesafterdrying.Liquidand
pastesamplesshouldbeevaporatedtodrynessona
steambath,then,asforthepowderedandgranular
forms,driedtoconstantweightat65°(see General
Provisions).
Assay Notlessthan6.1%totalnitrogen,whichisequivalent
tonotlessthan38.1%protein(%N×6.25),calculatedonthe
sodiumchloride-freebasis.

-AminoNitrogen/TotalNitrogen(AN/TN)PercentRa-
tio Notlessthan5.0%.
AmmoniaNitrogen Notmorethan1.0%,calculatedonthe
MicrobialLimits:
AerobicPlateCount Notmorethan50,000CFUper
gram.
Coliforms Notmorethan10CFUpergram.
Salmonella Negativein25g.
YeastsandMolds Notmorethan50CFUpergram.
Potassium Notmorethan13.0%.
SodiumChloride Notmorethan43.0%.
TESTS
Assay Determineasdirectedunder NitrogenDetermination,
AppendixIIIC.Calculatethepercentprotein(P)bythe
formula
P =6.25N,
inwhich N isthepercentnitrogen.

-AminoNitrogen/TotalNitrogen(AN/TN)PercentRa-
tio Determine -AminoNitrogen asdirectedunder -Amino
NitrogenDetermination,AppendixIIIC.Determine TotalNi-
trogen asdirectedunder NitrogenDetermination,Appendix
IIIC.CalculatetheAN/TNpercentratiobydividingtheper-
cent -aminonitrogen(AN)bythepercenttotalnitrogen(TN)
ascorrectedforammonianitrogen(NH3-N)accordingtothe
formula
100[(AN − NH3-N)/(TN − NH3-N)].
AmmoniaNitrogen Determineasdirectedunder Ammonia
Nitrogen,AppendixIIIC.
GlutamicAcid Determineasdirectedunder GlutamicAcid,
AppendixIIIC.
InsolubleMatter Transferabout5gofsample,accurately
weighed,intoa250-mLErlenmeyerflask.Add75mLof
water,covertheflaskwithawatchglass,andboilgentlyfor
2min.Filterthesolutionthroughataredfilteringcrucible,
dryat105°for1h,cool,andweigh.
Lead Determineasdirectedinthe FlameAtomicAbsorption
SpectrophotometricMethod under LeadLimitTest,Appendix
IIIB,usinga10-gsample.
Mercury Determineasdirectedunder MercuryLimitTest,
AppendixIIIB.
MicrobialLimits (Note: Currentmethodsforthefollowing
testsmaybefoundonlineat<www.cfsan.fda.gov/~ebam/
bam-toc.html>):
AerobicPlateCount
Coliforms
Salmonella
YeastsandMolds
Potassium1928